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The slots fill automatically by capillary action and then release the liquid to a surface or another liquid.
The typical arrayed material is of cDNA, DNA, RNA, oligos, PCR products, PCR digests, BAC libraries, YAC libraries, plasmid libraries, cosmid libraries, phage display libraries, antibodies, antigens and antibiotics.
By simply choosing the slot we can change the volume of liquid transported by Replicators and therefore the size of the spot.
Our standard Slot Pin Replicators have stainless steel 1.
Each pin transfers a precise amount of liquid because the size of the slot directly determines the volume of liquid transported.
We have standard slots of 0.
The 22 mm are for standard microplates and the 45 mm long slot pins are for deep well microplates.
To make slots that will yield the larger volumes we have to use pins of a larger diameter.
The end view of the slot is illustrated in photo to the right.
The 25 ul P11AS25 pins are 4.
We can also make that will deliver as little as 6 nl to meet your specific needs.
Also see our 8, 12, 16 and 24 and 8, 12, 16 and 24.
These are good for loading gels and making microarrays on glass slides.
The 22 mm long pins are better for loading or inoculating onto an agar surface.
Both of the pins are used to make quantitative liquid to liquid transfers or liquid to membrane blots.
VP 408S10 The VP 408S10 Multi-Blot Replicator in the photo to the right uses the P12S10 pin.
Please note that although the slots in the pin are a precise volume, the liquid that is transferred is usually more.
The reason for this is due to the surface tension of the liquid causing the liquid in the slot to "bow out" thus increasing the volume of the liquid in the slot.
If it is important for you to transfer exactly a certain volume, we can make to match the surface tension characteristics of your liquid.
Please visit this hyperlink for slot and pin on how to clean the slot pins 96 Slot Pin Multi-Blot Replicators 22 mm Long Slot Pins The nylon membrane on the right was blotted with a 5 ul Slot Pin Replicator from a microplate with serial 4 fold dilutions done in quadruplicate from blue and green dye.
Note that the size of the blot gets smaller with greater dilution.
This is because the dye actively binds to the membrane.
The size of the "wet blot" is the same in all the blots even though the color blots decrease in diameter with dilution.
This phenomena is due to the active binding of the dye to membrane as it wicks away from the slot pin.
Thus the bound material is concentrated at the contact point between the slot pin and the membrane and capture spot size grows only when the binding capacity of the membrane is exceeded.
Consequently, scientists can take advantage of this technique to concentrate small quantities of DNA or other binding substances onto a membrane.
As the concentration is diluted the charged membrane captures and contains it closer to the slot pin contact point, although the liquid solvent wicks from the slot pin and makes as large a spot as the undiluted concentration of dye seen in the photo on the left.
This solvent ring is not visible in the photo.
The concentration effect of the charged membrane and the solvent wicking action can be used to congratulate, free online slot machines with nudges and features apologise assay sensitivity.
Thus, high density arrays can be made where the solvent spots overlap each other but the DNA or dye spots do not.
The spot size diameter of aqueous solutions on a Nytran Super Charged Nylon Membrane are as follows: 0.
We highly recommend that you use the VP betfair casino promo codes Library Copier to assist in placing the replicator pins into the proper wells.
These are used primarily in arraying on glass slides.
For liquid to liquid delivery with slot pins we recommend using floating pin tools.
With the floating pin tools we have slot volumes that range from 10 nl to 50 nl.
This greatly simplifies loading the MADGE Gel wells with PCR product.
The Registration Block visually aligns with the 96 wells in the MADGE Gel.
Just select slot volume, pin length, pin diameter and pin format 48, 96, 384, 1536.
For comparison purposes we have included a standard paper clip in the photo.
Custom Light Weight Swiss Cheese Style Slot Pin Replicators One of our customers requested an ultra light weight Multi-Blot Replicator for a particular application so we designed a Replicator base with all of the excess aluminum removed.
We call it the "Swiss Cheese Style".
If you need a light Replicator just request a custom "Swiss Cheese.
You will come to appreciate a light weight replicator.
We can also make them with and with The advantage of floating slot pins is that the stainless steel pins have a precision slot cut into the tip of the pin to precisely pick up liquid via capillary action.
Each pin slides into a hole in a floating assembly until it "floats" on the gridding surface and delivers via capillary action, liquid from the slot at the tip.
Manual Multi-Blot Floating Slot Pin Replicators and Robot Floating Slot Pin Replicators are available in 96, 384, and 1536 formats.
The standard slot volumes for tube style pins available range from 6 nl to 500 nl.
A complete description of the floating slot pin replicators and how to order them is found on the The advantage of the E-Clip floating slot pins is that they carry a larger volume than the Tube style slot pins.
Each precisely picks up liquid via capillary action and delivers it on contact with a liquid or solid surface.
Each pin slides in a floating assembly until it "floats" on the gridding surface and delivers via capillary action, liquid from the slot at the tip.
This floating action assures that each of the pins will deliver its liquid even if the surface is not level betfair casino promo codes smooth.
Manual Multi-Blot Floating E-Clip Slot Pin Replicators and Robot Floating E-Clip Slot Pin Replicators are available in 96 and 384 formats.
The standard slot volumes for E-Clip style pins available range from 0.
This coating is metallic and only a few angstroms thick so it does not effect the basic dimensions of the pins.
These coated pins are very useful in handling solutions with proteins or lipids that may stick to naked stainless steel.
For an explanation see the and the Accessories We suggest you buy a few inexpensive accessories that will make the Replicators more useful, easier, safer and more reproducible.
This is very important as you do not slot and pin the pins to touch the side of wells as you remove the pins because that will knock off the hanging drop at the pin tip.
The is a registration device that allows you to make membrane arrays in an area the size of a standard microtiter plate with our Replicators.
Either four, 384 well plates or nine, 96 well plates can be arrayed into a 1536 or 864 array on the membrane.
After arraying from four, 384 well plates to one, 1536 membrane array betfair casino promo codes nine, 96 well plates to one, 864 membrane arraythe helps locate which mother plate well produced a positive spot on the membrane array.
The that is used in the sterilization of the Replicators by flaming.
Some labs use plastic reservoirs, but if flaming alcohol drops into them the plastic burns, dumping flaming alcohol all over the counter.
This covered dish is also useful for storing the alcohol as well as VP 110 Pin Cleaning Solution below for reuse.
The that is used to keep learn more here Replicator pins clean so they will carry the same amount of liquid each time.
Our that is used to clean and rotors slotted cross drilled treat the pin tips when they can't be flamed.
Integral to the cleaning process of all slot pins is the blotting step between baths.
Lint particles in the slots or pin tips, from blotting onto standard paper towels, will interfere with the loading and unloading of liquid.
The that doesn't transfer lint particles to the slot pins as betfair casino promo codes blotting papers or paper towels can do.
We strongly recommend that you use these blotting pads placed over several layers of paper towels.
These accessories are also found in the Basic Membrane Array Started Kit at a 5% discount.
Cleaning Pins The most important aspect of using pin tools is to start with clean pins and then keep them clean so they will consistently deliver the same volume.
We recommend our to clean the pin of protein and other residue buildup before you start an assay.
To quickly and efficiently remove the previous transfer from the pin we have a wide variety of manual and robot wash reservoirs ranging from disposable polypropylene reservoirs to reusable to clean 96, 384 and 1536 pin tools.
The robot wash stations are also adapted so the is part of the washing station thus reducing the cleaning cycle time and the number of robot stations required for the cleaning process.
We also have other and Integral to the cleaning process of all pins is the blotting step between baths.
Lint particles in the slots or on pin tips, from standard paper towels, will interfere with the loading and unloading of liquid.
We have solved this problem.
The blotting system is a tray with the standard microplate foot print that contains a super absorbent polypropylene pad covered by a Lint Free Blotting Paper.
We strongly recommend that you use this or our other We have prepared a detailed technical note which describes the step by step process of cleaning slot pins.
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If your power supply doesn't have a 6 pin PCI Express cable then you can use the adapter shown above on the right to convert two 4 pin peripheral cables into a PCI Express cable. If you use an adapter then be sure to plug the 4 pin peripheral connectors into separate cables coming from the power supply.
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